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09:21 min
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January 29, 2019
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Summary
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Aquí, describimos una estrategia eficiente y reproducible para producir, título y lotes de control de calidad de vectores de virus adeno-asociado. Permite al usuario obtener una preparación de vector con alto título (≥1 x 1013 vector genomas/mL) y una alta pureza, para uso en vitro o en vivo .
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Cite this Article
Fripont, S., Marneffe, C., Marino, M., Rincon, M. Y., Holt, M. G. Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors. J. Vis. Exp. (143), e58960, doi:10.3791/58960 (2019).
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