JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
利用多功能生化方法鉴定新型 ck2 激酶基材
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
Please note that all translations are automatically generated.
Click here for the English version.
利用多功能生化方法鉴定新型 ck2 激酶基材
DOI:
10.3791/59037-v
•
11:11 min
•
February 21, 2019
•
John E. Chojnowski
,
Emily A. McMillan
,
Todd I. Strochlic
1
Department of Biochemistry and Molecular Biology
,
Drexel University College of Medicine
Chapters
00:04
Title
00:35
Preparation of the Tissue Samples
01:20
Kinase Assay: Thiophosphorylation and Alkylation
02:34
Preparation of the Desalting Columns
03:40
Removal of PNBM
04:42
Immunoprecipitation: Part I
05:32
Protein A/G Agarose Bead Preparation
06:39
Immunoprecipitation: Part II
08:19
Analysis/Validation of Results
09:06
Results: Analysis and Identification of Novel CK2 Kinase Substrates
10:48
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议的目的是标记、丰富和识别复杂生物样品 (如细胞裂解物或组织均质) 中的蛋白激酶 ck2 底物。此方法利用 ck2 生物学的独特方面来实现这一目的。
Tags
CK2
Kinase Substrates
Biochemical Approach
Endogenous Substrates
Cell Lysate
GTPgammaS
PNBM
Sephadex G-25
Affinity Purification
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
A蛋白制备方法在蛋白质与核余子交互使用LC-MS / MS分析的高通量鉴定
使用硝酸纤维素结合分析细菌组氨酸激酶自身磷酸化的定量分析
用放射性标记的ATP测定蛋白激酶活性
碳水化合物转运基质结合蛋白 SP0092 的生化和结构表征
用
体外
激酶法鉴定细胞周期蛋白依赖性激酶1特异磷酸化部位
利用生物测定引导分馏法鉴定海洋鳗信息素
通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导
使用新激酶蛋白的强生化方法在分子水平上进行表征
自组装的人类蛋白质微阵列中的激酶抑制剂筛选
测量小核苷酸基质的多核苷酸磷酸化的非放射性测定
Read Article