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Chapters
- 00:04Title
- 00:40Fibrovascular Tissue Dissection
- 01:37Casting Upright Fibrin Gel Droplets for the Characterization of Native FT and Ex Vivo Culture
- 02:52Native FT and Ex Vivo Culture End-Point
- 03:19Whole-Mount Immunofluorescence Staining
- 07:00Results: PDR Ex Vivo Culture Induced Sprouting of CD31-positive Endothelium and Vasculature Preservation in Response to VEGFA
- 07:31Conclusion
Vi præsenterer her, en protokol til at studere Patofysiologi af proliferativ diabetisk retinopati ved hjælp af patient-afledte, kirurgisk skåret ud, fibrovascular væv for tre-dimensionelle indfødte karakterisering og ex vivo vævskultur. Dette ex vivo kultur model er også modtagelig for afprøvning eller udvikle nye behandlinger.
