This protocol describes how to establish an enteroid culture system from neonatal mouse or premature human intestine as well as an efficient method to collect milk from mice.
Lanik, W. E., Xu, L., Luke, C. J., Hu, E. Z., Agrawal, P., Liu, V. S., Kumar, R., Bolock, A. M., Ma, C., Good, M. Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation. J. Vis. Exp. (132), e56921, doi:10.3791/56921 (2018).