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Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation
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Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

DOI:
10.3791/56921-v

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09:02 min

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February 15, 2018

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, , , , , , , , ,
1Division of Newborn Medicine, Department of Pediatrics,Washington University School of Medicine, 2Washington University, 3Division of Newborn Medicine, Department of Pediatrics,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 00:57Crypt Isolation and Establishment of Enteroids
  • 04:08Mouse Breast Milk Collection
  • 05:03Staining
  • 07:35Results: Breast Milk Increases Growth and Proliferation of Enteroids
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to establish an enteroid culture system from neonatal mouse or premature human intestine as well as an efficient method to collect milk from mice.

Tags

Keywords: Breast MilkEnteroidsCell ProliferationEx VivoGastrointestinalNecrotizing EnterocolitisSmall IntestineCrypt IsolationBasement MembraneCell Disruption Media

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