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Cytometrische analyse van extracellulaire blaasjes van cel-geconditioneerd Media stromen
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Cytometrische analyse van extracellulaire blaasjes van cel-geconditioneerd Media stromen

Article DOI: 10.3791/59128-v • 08:32 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 1,2,3, 1
1Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, 3Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland
* These authors contributed equally

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Het protocol wordt een reproduceerbare methode ontworpen voor gebruik met cel cultuur supernatant voor het opsporen van oppervlakte epitopes op kleine extracellulaire blaasjes (EV) beschreven. Het maakt gebruik van specifieke EV immunoprecipitation met parels in combinatie met de antilichamen die surface antigeen CD9 herkent, CD63, en CD81. De methode is geoptimaliseerd voor downstream stroom cytometry analyse.

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Biologie kwestie 144 exosomes microvesicles stroom cytometry exosomes fenotypering kralen geconditioneerde gemiddeld extracellulaire blaasjes
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