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February 12, 2019
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Summary
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Het protocol wordt een reproduceerbare methode ontworpen voor gebruik met cel cultuur supernatant voor het opsporen van oppervlakte epitopes op kleine extracellulaire blaasjes (EV) beschreven. Het maakt gebruik van specifieke EV immunoprecipitation met parels in combinatie met de antilichamen die surface antigeen CD9 herkent, CD63, en CD81. De methode is geoptimaliseerd voor downstream stroom cytometry analyse.
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Cite this Article
Balbi, C., Bolis, S., Vassalli, G., Barile, L. Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media. J. Vis. Exp. (144), e59128, doi:10.3791/59128 (2019).
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