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Flow Cytometric analyse av ekstracellulære blemmer fra celle-conditioned Media
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Flow Cytometric analyse av ekstracellulære blemmer fra celle-conditioned Media

Article DOI: 10.3791/59128-v • 08:32 min • February 12th, 2019
February 12th, 2019 •
*1, *1, 1,2,3, 1
1Cellular and Molecular Cardiology Laboratory, Cardiocentro Ticino Foundation, Switzerland, 2Molecular Cardiology Institute, Dept. of Cardiology, University of Zürich, 3Faculty of Biomedical Science, Università Svizzera Italiana, Switzerland
* These authors contributed equally

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Protokollen beskriver en reproduserbar metode utviklet for bruk med celle kultur supernatants for å oppdage overflaten epitopes på små ekstracellulære blemmer (EV). Det bruker bestemte EV immunoprecipitation bruker perler kombinert med antistoffer som gjenkjenner overflate antigen CD9, CD63 og CD81. Metoden er optimalisert for nedstrøms flyt cytometri analyse.

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Biologi problemet 144 exosomes microvesicles flowcytometri exosomes phenotyping perler betinget medium ekstracellulære blemmer
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