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Direkte indsprøjtning af en Lentiviral vektor fremhæver flere Motor veje i rygmarven rotte
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
DOI:

07:57 min

March 15, 2019

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Chapters

  • 00:04Title
  • 00:45Surgical Preparation
  • 02:13Vertebral Column Exposure
  • 03:44Laminectomy
  • 04:36Virus Loading
  • 05:48Virus Injection and Post-Operative Care
  • 06:42Results: Representative Transduction of Neurons in the Spinal Cord and Brainstem
  • 07:31Conclusion

Summary

Automatic Translation

Denne protokol viser injektion af en retrogradely transportable viral vektor i rotte rygmarv væv. Vektor er taget på synapser og transporteres til cellen kroppen af target neuroner. Denne model er velegnet til retrograd sporing af vigtige spinal stier eller målretning celler for gen terapi programmer.

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