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Neuroscience
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直接注射慢病毒载体突出大鼠脊髓中的多种运动途径
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
Please note that all translations are automatically generated.
Click here for the English version.
直接注射慢病毒载体突出大鼠脊髓中的多种运动途径
DOI:
10.3791/59160-v
•
07:57 min
•
March 15, 2019
•
Kathleen M. Keefe
,
Ian P. Junker
,
Imran S. Sheikh
,
Thomas J. Campion
,
George M. Smith
1
Shriner’s Pediatric Research Center
,
Temple University
Chapters
00:04
Title
00:45
Surgical Preparation
02:13
Vertebral Column Exposure
03:44
Laminectomy
04:36
Virus Loading
05:48
Virus Injection and Post-Operative Care
06:42
Results: Representative Transduction of Neurons in the Spinal Cord and Brainstem
07:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议演示了在大鼠脊髓组织中注射一种具有可追溯性的可转移性病毒载体。载体在突触处被占据, 并被输送到目标神经元的细胞体。该模型适用于重要脊柱通路的逆行追踪或基因治疗应用中的靶向细胞。
Tags
Lentiviral Vector
Spinal Cord
Motor Pathways
Viral Vector
Genetic Material
Neural Circuits
Direct Injection
L1 To L4 Spinal Levels
Micromanipulator
Anesthetized Rat
Skin Incision
Spinous Processes
Rat Tooth Forceps
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