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使用基因激活 crispr 过度表达长非编码 rna
JoVE Journal
Genetics
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JoVE Journal
Genetics
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Please note that all translations are automatically generated.
Click here for the English version.
使用基因激活 crispr 过度表达长非编码 rna
DOI:
10.3791/59233-v
•
13:04 min
•
March 01, 2019
•
Carl Robert Rankin
,
Janet Treger
,
Emmanuelle Faure-Kumar
,
Jihane Benhammou
,
Deborah Anisman-Posner
,
Alex Edward Bollinger
,
Charalabos Pothoulakis
,
David Miguel Padua
1
Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine
,
University of California, Los Angeles
,
2
Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine
,
University of California, Los Angeles
,
3
Division of Hematology and Oncology, Department of Medicine
,
University of California, Los Angeles
Chapters
00:04
Title
00:40
Vector Design and Generation
01:52
Virus Generation
04:24
Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
07:07
dCas9 and gRNA Transduction, Selection, and Clone Creation
10:18
Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
12:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
传统的基于 cdna 的过度表达技术由于具有潜在功能的多个拼接形式, 对长非编码 rna 的过度表达的适用性有限。本综述报告了一个协议使用 crispr 技术超细的多个拼接变体的长非编码 rna。
Tags
Long Noncoding RNAs
Gene Activation
CRISPR
Isoforms
Genome
Endogenous Expression
Guide RNA
Deactivated Cas9
Lentivirus
HEK293T Cells
Transfection
Article
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