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使用基因激活 crispr 过度表达长非编码 rna
JoVE Journal
Genetics
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JoVE Journal Genetics
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
DOI:

13:04 min

March 01, 2019

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Chapters

  • 00:04Title
  • 00:40Vector Design and Generation
  • 01:52Virus Generation
  • 04:24Particle Count using p24 Enzyme-linked Immunosorbent Assay Kit
  • 07:07dCas9 and gRNA Transduction, Selection, and Clone Creation
  • 10:18Results: Using CRISPR Technology to Overexpress Multiple Splice Variants of a lncRNA
  • 12:08Conclusion

Summary

Automatic Translation

传统的基于 cdna 的过度表达技术由于具有潜在功能的多个拼接形式, 对长非编码 rna 的过度表达的适用性有限。本综述报告了一个协议使用 crispr 技术超细的多个拼接变体的长非编码 rna。

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