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Journal / Genetics / CRISPR の遺伝子活性化を使用して長い Rna…
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Genetics

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JoVE Journal Genetics
CRISPR の遺伝子活性化を使用して長い Rna を過剰発現
Identification of Circular RNAs using RNA Sequencing
Journal (Genetics)
Identification of Circular RNAs using RNA Sequencing
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Journal (Genetics)
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
 
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CRISPR の遺伝子活性化を使用して長い Rna を過剰発現

Article DOI: 10.3791/59233-v • 13:04 min • March 1st, 2019
March 1st, 2019 •
1, 1, 2, 1, 3, 3, 1, 1
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine, University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine, University of California, Los Angeles

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伝統的な cDNA を用いた発現技術の潜在的な機能を持つ、複数のスプライス フォームによる長鎖非コード Rna 発現限定適用しています。このレビューは、長鎖非コード RNA の複数のスプライスバリアント ノザンに CRISPR 技術を使用してプロトコルを報告します。

Tags

遺伝学、問題 145、lncRNA、CRISPR、IFNG AS1、過剰発現、レンチ ウイルス、リアルタイム PCR
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