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Imaging of Extracellular Vesicles by Atomic Force Microscopy
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Biochemistry
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JoVE Journal Biochemistry
Imaging of Extracellular Vesicles by Atomic Force Microscopy

Imaging of Extracellular Vesicles by Atomic Force Microscopy

DOI:
10.3791/59254-v

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10:11 min

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September 11, 2019

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,
1Department of Chemical Engineering,University of Utah, 2The Nano Institute of Utah,University of Utah, 3Center for Photonics and Quantum Materials,Skolkovo Institute of Science and Technology, 4Biopharmaceutical Cluster ‘Northern’,Moscow Institute of Physics and Technology

Chapters

  • 00:06Title
  • 01:03Surface Fixation of Extracellular Vesicles
  • 03:42AFM Imaging
  • 05:28Image Analysis
  • 07:55Results: Exosome Surface Fixation and Analysis
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties. 

Tags

Keywords: Extracellular VesiclesAtomic Force MicroscopyAFM ImagingElectrostatic ImmobilizationMica SurfaceHydrated SamplesDesiccated SamplesCryoTEMNanoparticle Tracking Analysis

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