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JoVE Journal
Biochemistry

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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
 

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Article doi: 10.3791/59750
July 20th, 2019

Summary July 20th, 2019

We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.

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