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JoVE Journal
Biochemistry
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Full Text
13,953 Views
09:21 min
July 20, 2019

DOI: 10.3791/59750-v

Minkyo Jung1, Ji Young Mun1

1Neural Circuits Research Group,Korea Brain Research Institute

We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.

This volume electron microscopy technique is used to perform biological studies that require the observation of a 3-D structure. Offering a rod, fielder view, and sample storage before image retakes. Fx2 Asan engineered the path stages that catalyzes the deabilation to increase the electron test of target structures and can be used to locate a specific target of interest.

These particles combines the use of correlative light and 3-D electron microscopy technique to investigate the interactions between membranous organelles and host cells. 16 to 24 hours after transfection, gently wash the culture with 250 microliters of 30 to 37 degree Celsius fixation solution. Quickly replacing the wash with 1.5 milliliters of fresh fixation solution, and placing the culture on ice for 30 minutes.

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