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黄荧光蛋白标记 p62 在攻击样诱导结构中光漂白后的荧光恢复
JoVE Journal
Biology
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JoVE Journal Biology
Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
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黄荧光蛋白标记 p62 在攻击样诱导结构中光漂白后的荧光恢复

DOI:
10.3791/59288-v

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12:58 min

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March 26, 2019

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, ,
1Core Imaging Facility and Department of Microbiology and Immunology,Loyola University Chicago, 2Department of Molecular Pharmacology and Therapeutics,Loyola University Chicago

Chapters

  • 00:04Title
  • 02:10Cell Treatments and Laser Setup
  • 03:44Image Acquisition Setup and Collection
  • 05:54Data Analysis
  • 08:08Data Analysis in a Spreadsheet Program
  • 10:16Results: Fluorescence Recovery After Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
  • 11:20Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种全面和实用的方法, 荧光恢复后的光漂白实验与活细胞。虽然该协议被用来测量黄色荧光蛋白标记 p62 在类似侵犯诱导结构中的流动性, 但它可以应用于各种显微镜系统和荧光蛋白。

Tags

Fluorescence Recovery After PhotobleachingFRAPYellow Fluorescent ProteinP62Aggresome-like Induced StructuresCell BiologyProtein MobilityDrug DeliveryDiffusionLive Cell ImagingMicroscopyRAW264.7 MacrophagesLipopolysaccharideNocodazoleTyrode’s Buffer514 Nm Laser

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