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Journal / Developmental Biology / In tempo reale Live-cella flusso Cytometry per…
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Developmental Biology

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JoVE Journal Developmental Biology
In tempo reale Live-cella flusso Cytometry per indagare l'afflusso del calcio, la formazione del poro e fagocitosi dai recettori P2X7 in cellule progenitrici neurali adulto
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In tempo reale Live-cella flusso Cytometry per indagare l'afflusso del calcio, la formazione del poro e fagocitosi dai recettori P2X7 in cellule progenitrici neurali adulto

Article DOI: 10.3791/59313-v • 11:47 min • April 3rd, 2019
April 3rd, 2019 •
1,2, 3,4, 3,5,6, 7, *1,4,7, *8
1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, 6School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science, Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health, University of Melbourne
* These authors contributed equally

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Fornire sensibilità unicellulare, citometria a flusso in tempo reale è in grado di quantificare le funzioni del ricevitore multimodale di culture vivono. Utilizzando cellule progenitrici neurali adulte, la funzione del recettore P2X7 è stata valutata tramite afflusso del calcio rilevato dal colorante indicatore di calcio, la formazione dei pori transmembrana dall'assorbimento di bromuro di etidio e fagocitosi utilizzando granuli di lattice fluorescente.

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Biologia dello sviluppo problema 146 recettori P2X7 citometria a flusso calcio segnalazione purinergic fagocitosi formazione dei pori cellule progenitrici neurali
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