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Elaborazione di protocollo per l'analisi della diffusione e dimensione dei Cluster di recettori di membrana mediante microscopia a fluorescenza
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1Department of Macromolecular Structures, Centro Nacional de Biotecnología,Campus Univ. Autónoma de Madrid, 2Campus Urb. Montepríncipe s/n,Univ. San Pablo CEU, 3Department of Immunology and Oncology, Centro Nacional de Biotecnología,Campus Univ. Autónoma de Madrid, 4Department of Cell Signaling,Centro Andaluz de Biología Molecular y Medicina Regenerativa (CSIC), 5Meyer Cancer Center, 6Department of Anatomy and Cell Biology,McGill Univ.
Chapters
- 00:04Title
- 01:37Preparation of Biological Samples and Image Acquisition
- 02:57Selection of Images and Creation of Masks
- 05:45Tracking Particles
- 08:09Calculation of the Diffusion Coefficients and Classification of Trajectories
- 09:10Calculation of Cluster Size
- 09:50Results: Analysis of Dynamic Fluorescence Particle Characteristics
- 11:24Conclusion
Qui, presentiamo un protocollo per singola particella monitoraggio analisi delle immagini che permette la valutazione quantitativa dei coefficenti di diffusione, tipi di movimento e cluster di dimensioni delle singole particelle rilevate mediante microscopia a fluorescenza.
