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Strålebehandling af Organotypiske kulturer fra Submandibulær og Parotid spytkirtler modeller Key in vivo egenskaber
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Strålebehandling af Organotypiske kulturer fra Submandibulær og Parotid spytkirtler modeller Key in vivo egenskaber

Article DOI: 10.3791/59484-v • 07:38 min • May 17th, 2019
May 17th, 2019 •
1, 2, 3, 1, 1,2
1Department of Nutritional Sciences, University of Arizona, 2Cancer Biology Graduate Interdisciplinary Program, University of Arizona, 3Department of Chemical Engineering, University of Arizona

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Ved hjælp af tre-dimensionelle organotypiske kulturer til at visualisere morfologi og funktionelle markører for spytkirtler kan give nye indblik i mekanismerne for vævsskade efter stråling. Beskrevet her er en protokol til sektion, kultur, irradiat, bejdsen, og billede 50 – 90 μm tykke spytkirtel sektioner før og efter udsættelse for ioniserende stråling.

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Medicin udstedelse 147 submandibulær spytkirtel vibratome kultur stråling parotideale spytkirtel organotypiske kultur xerostomien
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