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Vævs samling af flagermus til omics-analyser og primær cellekultur
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JoVE Journal Biology
Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Vævs samling af flagermus til omics-analyser og primær cellekultur

DOI:
10.3791/59505-v

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15:31 min

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October 23, 2019

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, , , , , , ,
1Department of Geology & Geophysics,Yale University, 2Department of Ecology & Evolution,Stony Brook University, 3Neurogenetics of Vocal Communication,Max Planck Institute for Psycholinguistics, 4School of Biological and Chemical Sciences,Queen Mary University of London, 5School of Biology & Environmental Science,University College Dublin, 6Donders Institute for Brain, Cognition and Behavior, 7Consortium for Inter-Disciplinary Environmental Research,Stony Brook University

Chapters

  • 00:04Title
  • 00:52Cranial Dissections for RNA
  • 04:05Postcranial Dissections for RNA
  • 06:24Tissue Culture
  • 09:41Refreshing Media and Passaging Cells
  • 11:38Conclusion
  • 13:15Freezing Viable Living Cells and Thawing the Frozen Cells
  • 14:48Results: Tissue Preparation and Cell Culture Results

Summary

Automatic Translation

Automatic Translation

Dette er en protokol for optimal vævs forberedelse til genomisk, transkriptomic og proteomiske analyser af flagermus fanget i naturen. Det omfatter protokoller for flagermus opsamling og dissektion, vævs bevaring, og celledyrkning af bat væv.

Tags

-omics AnalysesPrimary Cell CultureHumane SamplingRNA PreservationBrain DissectionOlfactory BulbCochleaSpecimen Identification

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