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Journal
/
Immunology and Infection
/
细胞培养和
果蝇黑色素
的氧化锌纳米颗粒的毒性研究
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in
Drosophila melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
细胞培养和
果蝇黑色素
的氧化锌纳米颗粒的毒性研究
DOI:
10.3791/59510-v
•
06:47 min
•
September 19, 2019
•
Cheng Teng Ng
,
Choon Nam Ong
,
Liya E. Yu
,
Boon Huat Bay
,
Gyeong Hun Baeg
1
NUS Environmental Research Institute & Department of Anatomy
,
National University of Singapore
,
2
NUS Environmental Research Institute & School of Public Health
,
National University of Singapore
,
3
Department of Civil and Environmental Engineering
,
National University of Singapore
,
4
Department of Anatomy
,
National University of Singapore
Chapters
00:04
Title
00:42
Fluorescence Activated Cell Sorting (FACS) Analysis on Lived/Fixed Cells
02:12
Exposure of ZnO NPs to
Drosophila
03:18
Dissection of Fly and ROS Detection
04:22
Measuring Fluorescence using ImageJ Software
05:03
Results: Toxicity Study of ZnO NPs
05:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个详细的方案,用于评估氧化锌纳米粒子(ZnO NPs)的毒理学特征,特别是人类MRC5肺成纤维细胞中的细胞死亡类型和果蝇
果蝇果蝇
的ROS形成。
Tags
Nanotoxicity
Cell Death
Reactive Oxygen Species
Fluorescence Activated Cell Sorting
DHE Probe
Zinc Oxide Nanoparticles
MRC5 Human Lung Fibroblasts
FITC Annexin V
Propidium Iodide
Flow Cytometry
Drosophila Melanogaster
Fly Food
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