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CRISPR/Cas9 Ribonucleoprotein-medierad exakt gen redigering av Tube Electroporation
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
DOI:

08:31 min

June 20, 2019

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Chapters

  • 00:04Title
  • 01:08Preparation of Cells
  • 02:51Tube Electroporation of Cas9 RNP and ssODNs
  • 04:24Analysis of Gene Editing Events
  • 05:48Results: Results of Tube Electroporation
  • 07:59Conclusion

Summary

Automatic Translation

Presenteras här är ett protokoll för effektiv CRISPR/Cas9 ribonucleoprotein-medierad gen redigering i däggdjurs celler med hjälp av rör elektroporation.

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