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可视化蛋白质激酶 在头部固定行为小鼠中使用 Vivo 双光子荧光寿命成像显微镜的活性
 

可视化蛋白质激酶 在头部固定行为小鼠中使用 Vivo 双光子荧光寿命成像显微镜的活性

Article doi: 10.3791/59526
June 7th, 2019

Summary June 7th, 2019

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提出了一个程序,以可视化蛋白激酶A活动在头部固定,行为小鼠。改进的A-激酶活动报告器tAKAR®在皮质神经元中表达,并通过颅窗口进行成像。双光子荧光寿命成像显微镜用于在强制运动期间可视化体内的PKA活动。

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