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Journal
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Immunology and Infection
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二次细菌性肺炎的鼠种模型的精确病原体输送与恢复系统
JoVE Journal
Immunology and Infection
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This content is Free Access.
JoVE Journal
Immunology and Infection
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
Please note that all translations are automatically generated.
Click here for the English version.
二次细菌性肺炎的鼠种模型的精确病原体输送与恢复系统
DOI:
10.3791/59566-v
•
00:13 min
•
September 21, 2019
•
Timothy R. Borgogna
,
Adrian Sanchez-Gonzalez
,
Kelly Gorham
,
Jovanka M. Voyich
1
Department of Microbiology and Immunology
,
Montana State University
,
2
University Communications
,
Montana State University
Chapters
00:01
Title
02:06
Intratracheal Instillation
04:46
Excision of Infected Lungs
07:16
Pathogen Recovery and Analysis
10:03
Results
12:06
Conclusion
Summary
Automatic Translation
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在这里,我们提出的方法,以改善继发性细菌性肺炎研究,通过提供非侵入性途径注入下呼吸道,然后病原体恢复和抄本分析。这些程序是可重复的,无需专用设备(如导管、导丝或光纤电缆)即可执行。
Tags
Secondary Bacterial Pneumonia
Murine Model
Pathogen Delivery
Pathogen Recovery
Tracheal Inoculation
Bacterial CFU Enumeration
RNA Purification
Virulence Gene Analysis
Minimally Invasive Technique
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