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制造淀粉样蛋白-β-分泌阿尔金酸微珠,用于模拟阿尔茨海默氏病
JoVE Journal
Biology
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JoVE Journal
Biology
Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease
Please note that all translations are automatically generated.
Click here for the English version.
制造淀粉样蛋白-β-分泌阿尔金酸微珠,用于模拟阿尔茨海默氏病
DOI:
10.3791/59597-v
•
06:52 min
•
July 06, 2019
•
Bushra Almari
,
David Brough
,
Michael Harte
,
Annalisa Tirella
1
Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health
,
University of Manchester
,
2
Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health
,
University of Manchester
Chapters
00:04
Title
01:02
Setting Up the Encapsulation System
02:16
Microbeads Fabrication
04:10
Testing and Use of Microbeads
04:57
Results: Testing of Amyloid–Secreting
06:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议说明了一种细胞封装方法,通过快速物理凝胶化藻酸盐来固定细胞。获得微珠允许随着时间的推移对淀粉样蛋白-α进行受控和持续分泌,并可用于研究在体外和体内的分泌淀粉样蛋白的影响。
Tags
Amyloid
Alginate Microbeads
Alzheimer’s Disease
Cell Encapsulation
Controlled Release
Biomolecule Release
Cell Suspension
Alginate Solution
Encapsulator
Gelation Bath
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