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JoVE Journal

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Article doi: 10.3791/59615
June 20th, 2019

Summary June 20th, 2019

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该协议描述了尺寸排除色谱与多角度光散射(SEC-MALS)的结合,用于对溶液中的蛋白质和复合物进行绝对表征。SEC-MALS 确定纯蛋白质、原生寡聚物、异质复合物和修饰性蛋白质(如糖蛋白)的分子量和大小。

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