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通过亲和色谱与西布洛特或质谱结合的亲致色谱,识别磷酸磷酸磷酸磷酸醇或磷酸蛋白
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Biochemistry
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JoVE Journal
Biochemistry
Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
通过亲和色谱与西布洛特或质谱结合的亲致色谱,识别磷酸磷酸磷酸磷酸醇或磷酸蛋白
DOI:
10.3791/59865-v
•
08:07 min
•
July 26, 2019
•
Igor Cestari
1
Institute of Parasitology
,
McGill University
Chapters
00:04
Title
00:58
Cell Growth, Lysis, and Affinity Chromatography
04:27
Results: Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins
06:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议侧重于识别与磷酸肌醇或磷酸磷酸剂结合的蛋白质。它使用亲和色谱与生物异化异酸醇磷酸盐或磷酸磷酸酯,通过链球菌固定到胶质或磁珠。磷酸磷酸磷酸磷酸磷酸磷酸二醇结合蛋白通过西方印迹或质谱法鉴定。
Tags
Inositol Phosphate
Phosphoinositide
Protein Binding
Affinity Chromatography
Western Blot
Mass Spectrometry
Regulatory Function
Signal Transduction
Cell Development
Biotinylated
Lyposome Free
T. Brucei
PBS-G
Lysis Buffer
Protease Inhibitor
Phosphatase Inhibitor
Binding Assay
Article
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