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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro transkriberet RNA-baseret luciferase reporter assay til undersøgelse oversættelse forordning i poxvirus-inficerede celler
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In vitro transkriberet RNA-baseret luciferase reporter assay til undersøgelse oversættelse forordning i poxvirus-inficerede celler

Article DOI: 10.3791/59626-v • 08:58 min • May 1st, 2019
May 1st, 2019 •
1, 1, 1, 1
1Division of Biology, Kansas State University

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Vi præsenterer en protokol for at studere mRNA-oversættelses regulering i poxvirus-inficerede celler ved hjælp af in vitro transskriberet RNA-baseret luciferase reporter assay. Analysen kan bruges til at studere oversættelse regulering af CIS-elementer i en mRNA, herunder 5 '-uoversat region (UTR) og 3 '-UTR. Forskellige oversættelses Initierings tilstande kan også undersøges ved hjælp af denne metode.

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Immunologi og infektion in vitro transkription luciferase assay vaccinia virus poxvirus oversættelse 5 '-UTR 5 '-poly (A) leder
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