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JoVE Journal
Immunology and Infection

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Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
 

Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells

Article DOI: 10.3791/62984-v
October 19th, 2021

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Summary October 19th, 2021

Metabolic adaptation is fundamental for T cells as it dictates differentiation, persistence, and cytotoxicity. Here, an optimized protocol for monitoring mitochondrial respiration in ex vivo cytokine-differentiated human primary T cells is presented.

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