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Immunology and Infection

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JoVE Journal Immunology and Infection
体外转录 rna 基荧光素酶记者法研究类病毒感染细胞的翻译规律
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
Journal (Immunology and Infection)
Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
Collection, Embedding, Sectioning, and Staining of the Mouse Submandibular Salivary Gland for Live Cell Imaging of Cell-Cell Interactions (Video) | JoVE
Journal (Immunology and Infection)
Collection, Embedding, Sectioning, and Staining of the Mouse Submandibular Salivary Gland for Live Cell Imaging of Cell-Cell Interactions (Video) | JoVE
 
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体外转录 rna 基荧光素酶记者法研究类病毒感染细胞的翻译规律

Article DOI: 10.3791/59626-v • 08:58 min • May 1st, 2019
May 1st, 2019 •
1, 1, 1, 1
1Division of Biology, Kansas State University

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我们提出了一种研究 mrna 翻译调节的方案, 在天花病毒感染细胞使用体外转录 Rna 基荧光素酶报告法。该方法可用于研究 mRNA 的cis元素的翻译调控, 包括 5 '-未翻译区域 (utr) 和 3 '-utr。使用此方法还可以检查不同的翻译启动模式。

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免疫学与感染 第147期 体外转录 荧光素酶检测 疫苗病毒 Poxvirus 翻译 5 '-utr 5 '-聚 (A) 领导者
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