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Effektiv transfektion af in vitro transkriberet mRNA i dyrkede celler ved hjælp af peptid-poloxamin nanopartikler
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Immunology and Infection
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JoVE Journal Immunology and Infection
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Effektiv transfektion af in vitro transkriberet mRNA i dyrkede celler ved hjælp af peptid-poloxamin nanopartikler

DOI:
10.3791/64288-v

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10:16 min

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August 17, 2022

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, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Department of Pediatrics,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Chapters

  • 00:05Introduction
  • 01:19In Vitro Transcription (IVT) of Chemically Modified mRNA
  • 02:55Generation of IVT mRNA/PP-sNps
  • 05:22Preparation of the Cells for Transfection and Transfection of the Cultured Cells
  • 07:04Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay
  • 07:53Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

En selvsamlet peptid-poloxamin nanopartikel (PP-sNp) er udviklet ved hjælp af en mikrofluidisk blandeanordning til at indkapsle og levere in vitro transkriberet messenger RNA. Det beskrevne mRNA/PP-sNp kunne effektivt transfektere dyrkede celler in vitro.

Tags

MRNA TransfectionPeptide-poloxamine NanoparticlesMucosal MRNA VaccineLung CancerCystic FibrosisIn Vitro TranscriptionCapped MRNAAgarose Gel ElectrophoresisPoloxamine 704Synthetic Peptide

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