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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro transkribere RNA-basert luciferase reporter analysen å studere oversettelse regulering i Poxvirus-infiserte celler
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In vitro transkribere RNA-basert luciferase reporter analysen å studere oversettelse regulering i Poxvirus-infiserte celler

Article DOI: 10.3791/59626-v • 08:58 min • May 1st, 2019
May 1st, 2019 •
1, 1, 1, 1
1Division of Biology, Kansas State University

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Vi presenterer en protokoll for å studere mRNA oversettelse regulering i poxvirus celler med in vitro transkribere RNA-basert luciferase reporter analysen. Analysen kan brukes til å studere oversettelse regulering av CIS-elementer av en mRNA, inkludert 5 '-uoversatt region (UTR) og 3 '-UTR. Ulike oversettelse initiering moduser kan også undersøkes ved hjelp av denne metoden.

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Immunologi og infeksjon in vitro transkripsjon luciferase analysen Vaccinia virus Poxvirus oversettelse 5 '-UTR 5 '-Poly (A) leder
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