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En in vitro-protokol til evaluering af MicroRNA-niveauer, funktioner og tilknyttede Målgener i tumor celler
JoVE Journal
Genetics
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JoVE Journal Genetics
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
DOI:

09:45 min

May 21, 2019

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Chapters

  • 00:04Title
  • 01:06MicroRNA (miRNA) Mimic Transfection
  • 02:03Sulforhodamine B (SRB) Assay
  • 03:20Generation of a Dose-response Curve
  • 04:35Verification of the Direct Target Gene of a miRNA of Interest
  • 06:37Luciferase Assay
  • 07:52Results: miRNA-107 Negatively Regulates Proliferation of PANC-1 Cells
  • 08:53Conclusion

Summary

Automatic Translation

Denne protokol bruger en Probe-baseret real-time polymerase kædereaktion (PCR), en sulforhodamin B (SRB) assay, 3 ' ikke oversat regioner (3 ' UTR) kloning, og en der analyse for at verificere målgener af en Mirna af interesse og til at forstå funktionerne i Mirnas.

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