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利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境
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Cancer Research
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JoVE Journal Cancer Research
Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
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利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境

DOI:
10.3791/59633-v

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09:42 min

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June 26, 2019

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, , , , , , ,
1Department of Anatomy and Structural Biology,Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine, 3Integrated Imaging Program,Albert Einstein College of Medicine, 4Department of Surgery,Montefiore Medical Center, 5Department of Pathology,Montefiore Medical Center

Chapters

  • 00:04Title
  • 00:55Transplantation of Syngeneic Breast Tumors into Mouse Hosts with Fluorescent Macrophages
  • 02:38Preparation of Mouse for Imaging
  • 04:25Evaluation of Extravascular Dextran Using Fixed Tissue Analysis
  • 07:10Results: Visualization of Vasculature in Healthy and Diseased Tissues and TMEM-Mediated Vascular Permeability
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了两种方法,用于评估与转移(TMEM)的肿瘤微环境(TMEM)和癌细胞内溢相关的瞬态血管渗透性,使用静脉注射高分子量(155 kDa)dextran小鼠。这些方法包括活体动物的活体成像和使用免疫荧光的固定组织分析。

Tags

Tumor MicroenvironmentTMEMVascular PermeabilityCancer Cell DisseminationIntravital ImagingDextranTumor TransplantationMacrophagesMammary Fat PadExtracellular MatrixTail Vein Catheter

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