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Bioengineering
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用于长期无细胞基因表达传导的多层微流体平台
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Please note that all translations are automatically generated.
Click here for the English version.
用于长期无细胞基因表达传导的多层微流体平台
DOI:
10.3791/59655-v
•
11:23 min
•
October 06, 2019
•
Ardjan J. van der Linden
,
Maaruthy Yelleswarapu
,
Pascal A. Pieters
,
Zoe Swank
,
Wilhelm T. S. Huck
,
Sebastian J. Maerkl
,
Tom F. A. de Greef
2
1
Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group
,
Eindhoven University of Technology
,
2
Institute for Molecules and Materials
,
Radboud University
,
3
Institute of Bioengineering
,
School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
Chapters
00:04
Title
01:00
Off-Chip Cooling Setup
02:35
Preparing the Setup for an Experiment
04:20
Connecting Uncooled Reagents to the Device
05:45
Connecting Cooled Fluids to the Microfluidic Device
06:49
Loading the Microfluidic Device
08:09
Calibrating the Microfluidic Device
08:56
Results: IVTT Experiment Expressing the deGFP Protein
10:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
介绍了基于PDMS的多层微流体装置的制造过程,该装置允许长时间进行体外转录和翻译(IVTT)反应。此外,还全面概述了自动化和维护这些反应所需的硬件和软件,
Tags
Microfluidic Platform
Cell-free Gene Expression
Synthetic Gene Networks
Prototyping
GFP
Biochemical Reactions
Flow Reactor
Pneumatic Control
Peltier Cooling
PTFE Tubing
PEEK Tubing
Temperature Control
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