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JoVE Journal Developmental Biology
Expressão rápida e eficiente de múltiplas proteínas em embriões aviários usando o mRNA Electroporation
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Expressão rápida e eficiente de múltiplas proteínas em embriões aviários usando o mRNA Electroporation

Article DOI: 10.3791/59664-v • 09:40 min • June 7th, 2019
June 7th, 2019 •
1, 2,3, 1,2,3
1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

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Nós relatamos o electroporation do RNA do mensageiro (mRNA) como um método que permita a expressão rápida e eficiente de proteínas múltiplas no sistema do modelo do embrião de codorniz. Este método pode ser usado para cDNAs etiquetar pilhas e gravar seus movimentos in vivo pela microscopia do tempo-lapso logo após o electroporation.

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Biologia do desenvolvimento edição 148 eletroporação de mRNA microscopia de lapso de tempo codorniz aviária embrião FRAP
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