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Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
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1Department of Biological Sciences,University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute,Children’s Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology,University of Southern California, Los Angeles
Chapters
- 00:04Title
- 00:55Living Quail Embryo Preparation
- 02:50Electroporation-encoded Fluorescent Protein (FP) Imaging
- 05:44Fluorescence Recovery After Photobleaching (FRAP)
- 07:35Results: Representative Electroporation-encoded FP Expression in Avian Embryos
- 09:21Conclusion
We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.
