01:33Lentivirus-Renilla-Luciferin-Red Fluorescent Protein (LV-Rluc-RFP; RR) and LV-Rluc-RFP-Herpes Simplex Virus Truncated Thymidine Kinase (HSV-ttk) (LV-Rluc-RFP-TTK; RT) Packaging and Production
03:38LV-RR and LV-RRT Lentiviral Transduction for Gene Expression in 4T1 Cells
04:37Drug Screening and LV-RR and LV-RRT-Transduced 4T1 Cell Identification
05:18Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)-Firefly Luciferase (Fluc)-Knockin (KI) Mice and Tumor-Bearing Mouse Model
06:50Dual Bioluminescence Tumor (Rluc) and Angiogenesis (Fluc) Imaging
07:58Results: Representative In Situ Tumor Growth and Angiogenesis Visualization and Analysis
Bu protokol, Çift Bioluminesans görüntüleme ile tümör ilerlemesini ve anjiyogenezi gerçek zamanlı olarak izlemek için tümör taşıyan bir fare modelinin kurulması açıklanmaktadır.