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Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging
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Medicine
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JoVE Journal Medicine
Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

DOI:
10.3791/2775-v

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08:04 min

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April 14, 2011

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, , , , ,
1Imaging Biology Research and Development,Caliper Life Sciences

Chapters

  • 00:05Title
  • 01:26Intracardiac Injection of Cells into Animals
  • 02:58Bioluminescence Imaging to Monitor Metastases
  • 03:56Longitudinal Monitoring of Osteolytic Lesions Using Micro CT
  • 05:023D Co-registration and Analysis of BLI and Micro CT
  • 05:39Monitoring Tumor Metastases and Osteolytic Lesions after Intracardiac Injection
  • 07:30Conclusion

Summary

Automatic Translation

Automatic Translation

An experimental mouse model of bone metastasis was established following intracardiac delivery of luciferase expressing mammary tumor cells. Tumor development and resulted osteolytic lesion were monitored longitudinally with bioluminescence and micro CT imaging.

Tags

Tumor MetastasesOsteolytic LesionsBioluminescence ImagingMicro CT ImagingMDA-MB-231-luc-D3H2LN CellsNu/Nu MiceIntracardiac DeliverySystemic MetastasesIVIS SpectrumDLIT ReconstructionTumor SignalTibiaFemurMicro CT SystemQuantum FXX-ray DoseRadiation DoseLD50Mouse Imaging Shuttle DeviceAnimal PositioningCo-registrationLiving Image 4.1 Software

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