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Journal / Bioengineering / 在肽膜前体的维西图洛合成中,用于自主囊泡生长…
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Bioengineering

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JoVE Journal Bioengineering
在肽膜前体的维西图洛合成中,用于自主囊泡生长
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在肽膜前体的维西图洛合成中,用于自主囊泡生长

Article DOI: 10.3791/59831-v • 07:10 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 2, 2, 1,3, 1
1Physics of Synthetic Systems - E14, Physics-Department and ZNN, Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM), Technische Universität München, 3Nanosystems Initiative Munich

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这里介绍了创建能够生长的基于肽的小型单片杆菌囊泡的协议。为了便于膜肽的囊泡生产,这些囊泡配备了转录翻译系统和肽编码质粒。

Tags

生物工程,第148期,合成细胞,乳酸样多肽,囊泡生长,囊泡肽表达,封装,膜生长,转录翻译系统
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