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大小排除色谱分析细菌外膜囊性异质性
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
DOI:

07:26 min

March 31, 2021

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Chapters

  • 00:04Introduction
  • 00:53S-1000 Column Packing
  • 02:22Sample Loading and Fraction Collection
  • 03:52Sample Analysis
  • 05:51Results: Analysis of Lipid and Toxin Contents of the Collected Fractions
  • 06:51Conclusion

Summary

Automatic Translation

细菌囊泡在发病机制中起着重要作用,具有广阔的生物技术应用前景。囊泡的异质性使分析和使用复杂化:因此,需要一种简单、可重复的方法来分离不同大小的囊泡。在这里,我们演示了使用大小排除色谱来分离由 聚合菌作用肌瘤产生的异质囊泡。

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