JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
生物应用近红外发光金纳米簇的合成
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Please note that all translations are automatically generated.
Click here for the English version.
生物应用近红外发光金纳米簇的合成
DOI:
10.3791/60388-v
•
09:11 min
•
March 22, 2020
•
Goutam Pramanik
,
Alena Keprova
,
Jan Valenta
,
Vaclav Bocan
,
Klaudia Kvaková
4
,
Lenka Libusova
,
Petr Cigler
1
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences
,
2
Department of Chemical Physics and Optics, Faculty of Mathematics and Physics
,
Charles University
,
3
Department of Cell Biology, Faculty of Science
,
Charles University
,
4
First Faculty of Medicine
,
Charles University
Chapters
00:05
Introduction
01:10
Preparation of Near-infrared Emitting AuNCs
02:13
Conjugation of TPP on the Surface of 1
02:55
AuNC Internalization into HeLa Cells
03:55
Flow Cytometry Analysis
05:26
CLSM Imaging of Live HeLa Cells Labeled with 2
07:15
Results: Gold Nanoclusters inside HeLa Cells
08:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
介绍了一种可靠且易于重现的方法,用于制备可功能的近红外发光金纳米簇,并通过流细胞学和共聚焦激光扫描显微镜直接检测HeLa细胞内部。
Tags
Gold Nanoclusters
Near-infrared Emitting
Photoluminescent
Thiol-functionalized Ligand
Amine-functionalized Ligand
Biosensing
Bio-imaging
Ultrafiltration
Polyethylene Glycol
EDC HCL
Cell Culture
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
单层的合成,装配和表征蛋白质单分子膜的电化学保护的金纳米粒子薄膜
黄金Nanostar合成银种子介导的生长方法
宽带超声医疗成像应用,光学检测仪
就地
液体生物组件的 TEM
荧光淬一个脂质体包封的近红外荧光团的作为一个工具<em>在体内</em>光学成像
基于角蛋白纳米纤维生物医学工程合成
从混合三维培养使用近红外Photoimmunotherapy技术选择性细胞消除
硅纳米线场效应晶体管的研制化学生物传感与应用
RGD功能化水凝胶作为治疗应用的工具合成
水凝胶的合成与防污性能作为膜的净水
Read Article