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Ultrasonic-augmented Primary Adult Fibroblast Isolation
JoVE Journal
Medicine
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JoVE Journal Medicine
Ultrasonic-augmented Primary Adult Fibroblast Isolation
DOI:

06:51 min

July 29, 2019

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Chapters

  • 00:04Title
  • 00:40Mouse Dissection and Organ Removal
  • 02:12Tissue Mincing and Digestion and Cell Extraction
  • 04:49Results: Representative Differentiated Fibroblast Characterization
  • 06:19Conclusion

Summary

Automatic Translation

We present a protocol to isolate primary adult fibroblasts in an easy, fast and reliable way, performable by beginners (e.g., students). The procedure combines enzymatic tissue digestion and mechanical agitation with ultrasonic waves to obtain primary fibroblasts. The protocol can easily be adapted to specific experimental requirements (e.g., human tissue).

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