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Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
JoVE Journal
Medicine
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JoVE Journal Medicine
Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

DOI:
10.3791/62514-v

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06:27 min

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May 13, 2021

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, , , , , , , , , , ,
1Department of Cardiology, Bejing Anzhen Hospital,Capital Medical University, 2Department of Cardiology,Bejing Chuiyangliu Hospital

Chapters

  • 00:05Introduction
  • 00:38Heart Excision and Aortic Cannulation
  • 02:38Heart Perfusion
  • 03:37Cell Isolation and Calcium Reintroduction
  • 04:46Results: Survival Rates and Quality of the Isolated Cardiac Myocytes
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes modifications of the Langendorff method including the depth of aorta cannulation for simultaneous Isolation of atrial and ventricular myocytes from adult mice.

Tags

Keywords: Langendorff MethodAtrial MyocytesVentricular MyocytesAdult MiceSimultaneous IsolationHeart DiseaseCellular MechanismsEuthanasiaAortic CannulationLangendorff ApparatusEnzymatic DigestionCell Isolation

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