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Genetics
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人类胰腺胰岛的单细胞RNA测序与分析
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Genetics
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JoVE Journal
Genetics
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
Please note that all translations are automatically generated.
Click here for the English version.
人类胰腺胰岛的单细胞RNA测序与分析
DOI:
10.3791/59866-v
•
11:34 min
•
July 18, 2019
•
Yurong Xin
,
Christina Adler
,
Jinrang Kim
,
Yueming Ding
,
Min Ni
,
Yi Wei
,
Lynn Macdonald
,
Haruka Okamoto
1
Regeneron Pharmaceuticals, Inc
Chapters
00:04
Title
01:01
Human Islet Dissociation
02:30
Single Cell Suspension Quality Control and Single Cell Partitioning
04:10
Single Cell cDNA Amplification
06:19
Library Sequencing
08:11
Read Alignment and Data Analysis
09:53
Results: Dissociation, Single-cell Emulsion, cDNA Library, and Cell Types
11:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,利用基于液滴的微流体单细胞RNA测序技术,从分离的人类胰腺胰岛生成单个细胞的高质量、大规模转录组数据。
Tags
Single-cell RNA Sequencing
Human Pancreatic Islets
Cell Heterogeneity
Rare Cell Type
Gene Regulation
Dissociation
Cell Suspension
Emulsion
Cell Count
Cell Viability
Microfluidic Chip
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