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Journal
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Biochemistry
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淀粉样蛋白纤维蛋白的相互作用和脑线粒体的膜渗透
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Biochemistry
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Biochemistry
Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
Please note that all translations are automatically generated.
Click here for the English version.
淀粉样蛋白纤维蛋白的相互作用和脑线粒体的膜渗透
DOI:
10.3791/59883-v
•
00:15 min
•
September 28, 2019
•
Ramin Zadali
,
Ebrahim Rostampour Ghareghozloo*
1
,
Mohammad Ramezani*
1
,
Vahid Hassani*
1
,
Yasin Rafiei*
1
,
Shiva Mohammadi Chiyaneh
,
Ali Akbar Meratan
1
Department of Biological Sciences
,
Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran
Chapters
00:00
Title
01:10
Brain Homogenization and Mitochondrial Isolation
04:33
Mitochondrial Membrane Integrity Determination
05:58
Malate Dehydrogenase Activity Determination
07:07
In vitro α-synuclein, Bovine Insulin, and HEWL Fibril Formation
10:11
Treatment of Mitochondria with Amyloid Fibrils, MDH Release Assay, and ROS Measurement
12:14
Results
14:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里提供了一个协议,用于研究从不同组织和大脑不同区域分离的来自不同组织和不同区域的线粒体的不同肽和蛋白质的原生形态、前纤维和成熟的淀粉样纤维之间的相互作用。
Tags
Mitochondria
Amyloid Fibrils
Alpha-synuclein
Membrane Permeabilization
Neurodegenerative Diseases
Brain
Isolation
Homogenization
Centrifugation
Density Gradient
Myelin
Mitochondrial Fraction
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