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使用点击化学测量血管平滑肌细胞增殖
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Biology
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JoVE Journal
Biology
Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry
Please note that all translations are automatically generated.
Click here for the English version.
使用点击化学测量血管平滑肌细胞增殖
DOI:
10.3791/59930-v
•
07:17 min
•
October 30, 2019
•
Victoria Wong
,
Prakash Arumugam
,
Lucile E. Wrenshall
2
1
Department of Neuroscience, Cell Biology, and Physiology
,
Wright State University
,
2
Department of Surgery, Boonshoft School of Medicine
,
Wright State University
Chapters
00:05
Title
00:30
Vascular Smooth Muscle Cell Labelling with EdU and Fixing with PFA
01:53
Detection of EdU Using Fluorescence
02:50
Detection of EdU Using Luminescence
04:40
Results: Proliferation of Vascular Smooth Muscle Cells Measurements
06:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
增殖是细胞功能的关键部分,也是用于评估新药潜在毒性的常见读物。因此,测量增殖是细胞生物学中常用的测定方法。在这里,我们提出了一种简单、通用的测量增殖方法,可用于附着细胞和非粘附细胞。
Tags
Vascular Smooth Muscle Cells
Cell Proliferation
Click Chemistry
EdU
Fluorescence
Luminescence
Cell Assay
PDGF
Paraformaldehyde
TX-100
THPTA
Copper Sulfate
Fluorescein Picolyl-azide
Sodium Ascorbate
DAPI
HRP Azide
ELISA Substrate
Article
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