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Journal / Immunology and Infection / Anvendelse af et influenza antigen-Mikroarray til …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Anvendelse af et influenza antigen-Mikroarray til måling af bredden af serum antistoffer på tværs af virus undertyper
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Anvendelse af et influenza antigen-Mikroarray til måling af bredden af serum antistoffer på tværs af virus undertyper

Article DOI: 10.3791/59973-v • 08:52 min • July 26th, 2019
July 26th, 2019 •
1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2
1Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, 2Vaccine Research and Development Center, Department of Physiology, University of California Irvine

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Vi præsenterer en protokol for anvendelse af et protein mikrosystem, der er konstrueret ved at udskrive influenza antigener på nitrocellulose belagte slides til samtidig sonde serum for flere antistof isotyper mod over 250 antigener fra forskellige virusstammer, således tillader måling af bredden af serumantistoffer på tværs af virusundertyper.

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Immunologi og infektion protein mikroarray influenza virus antigen antistof hemagglutinin immunitet
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