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JoVE Journal
Biochemistry

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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应
 
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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应

Article doi: 10.3791/60017
August 17th, 2019

Summary August 17th, 2019

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一种烟酰胺腺苷二核苷酸(NADH)与ATPase的检测已应用于小分子肌苷抑制剂的半高通量筛选。此动力学测定以 384 孔微孔板格式运行,总反应体积仅为每孔 20 μL。该平台应适用于几乎任何ADP生产酶。

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