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Journal / Genetics / Single-molekyle Förster Resonance…
JoVE Journal
Genetics

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JoVE Journal Genetics
Single-molekyle Förster Resonance energioverførsel metoder til real-time undersøgelse af Holliday Junction resolution af GEN1
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Single-molekyle Förster Resonance energioverførsel metoder til real-time undersøgelse af Holliday Junction resolution af GEN1

Article DOI: 10.3791/60045-v • 11:27 min • September 18th, 2019
September 18th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)
* These authors contributed equally

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Præsenteret her er en protokol til udførelse af Single-molekyle Förster Resonance energioverførsel til at studere HJ opløsning. To-farve alternerende excitation bruges til bestemmelse af dissociations konstanter. Enfarvet tidsforskydning smFRET påføres derefter i realtid spaltning assays for at opnå den pause tidsfordeling før HJ opløsning.

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Genetik Holliday Junction enkelt-molekyle fret homologe rekombination 5 ' nukleaser Gap endonuklease I GEN1 Holliday Junction resolvases
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