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Human Egg Maturity Assessment and Its Clinical Application
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Human Egg Maturity Assessment and Its Clinical Application

Human Egg Maturity Assessment and Its Clinical Application

DOI:
10.3791/60058-v

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08:51 min

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August 19, 2019

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, , , , ,
1Reprofit International,Clinic of Reproductive Medicine, 2Department of Histology and Embryology, Faculty of Medicine,Masaryk University

Chapters

  • 00:04Title
  • 01:13Egg Retrieval and Denudation
  • 02:24Dish Preparation
  • 04:17Polarized Light Microscopy (PLM) Examination Preparation
  • 05:23Egg Maturity Examination
  • 06:25Intracytoplasmic Sperm Injection (ICSI) Timing Optimization
  • 07:35Results: Metaphase I (MI) to MII Transition Stages in Oocyte Maturation
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

We provide an outline of the clinical protocol for non-invasive assessment of human egg maturity using polarized light microscopy.

Tags

Keywords: Egg Maturity AssessmentPolar BodyMeiotic SpindleICSIIVFOocyteCumulus-oocyte ComplexesHyaluronidaseCulture MediumCarbon Dioxide Incubator

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