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Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

DOI:
10.3791/57641-v

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08:32 min

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March 27, 2019

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, , ,
1Center for iPS Cell Research and Application (CiRA),Kyoto University

Chapters

  • 00:04Title
  • 00:28Prepare Basement Membrane Matrix-coated Plates
  • 01:15Seed the hPSCs and Initiate Differentiation to Definitive Endoderm
  • 03:57Induce the Differentiation into Definitive Endoderm
  • 05:13Induce the Differentiation into the Primitive Gut Tube
  • 05:47Induce the Differentiation into PDX1+ Cells
  • 06:28Results: Analysis of hPSCs and the Generated Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a detailed protocol to differentiate human pluripotent stem cells (hPSCs) into pancreas/duodenum homeobox protein 1+ (PDX1+) cells for the generation of pancreatic lineages based on the non-colony type monolayer growth of dissociated single cells. This method is suitable for producing homogenous hPSC-derived cells, genetic manipulation and screening.

Tags

Keywords: HPSCsAdhesion CultureBasement Membrane MatrixEDTAY27632Cell DissociationCell CountingStage 1A MediumPancreatic Progenitors

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