A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:58Electrospray-ion Mobility-Mass Spectrometry Analysis
- 03:02Analysis of ESI-IM-MS pH Titration Data
- 06:11Collision Cross-Sections and Computational Methods
- 08:30Results: Reaction Mechanisms and Coordination Sites of the Alternative Methanobactin Complexes
- 10:00Conclusion
Jon mobilitet-masspektrometri och molekylära modelleringstekniker kan karakterisera den selektiva metallkelaterande prestandan hos designade metallbindande peptider och den kopparbindande peptiden methanobactin. Utveckla nya klasser av metallkelerande peptider kommer att bidra till att leda till Therapeutics för sjukdomar som förknippas med metall Jon obalans.
Tags
Ion Mobility-mass SpectrometryIM-MSMetal Ion RecognitionRedox ActivityMetal Binding OligopeptidesMolecular ModelingTertiary StructureMetal CorrelationESI-IM-MSPoly-DL-alanineAlternative MethanobactinPH-dependent RedoxMethyl Binding ReactionProduct IonsStoichiometryProtonation StateConformational StructureMetal Chelating PeptidesTherapeuticsMenkes DiseaseWilson’s DiseaseCancerAlzheimer’s DiseaseNative ESI-IM-MSNegative IonPositive IonAcetic AcidAmmonium HydroxideMass-to-chargeIsotope Pattern
